Polyclonal Proliferation and Apoptosis of CCR5+ T Lymphocytes during Primary Human Immunodeficiency Virus Type 1 Infection: Regulation by Interleukin (IL)-2, IL-15, and Bcl-2
Identifieur interne : 00AE58 ( Main/Exploration ); précédent : 00AE57; suivant : 00AE59Polyclonal Proliferation and Apoptosis of CCR5+ T Lymphocytes during Primary Human Immunodeficiency Virus Type 1 Infection: Regulation by Interleukin (IL)-2, IL-15, and Bcl-2
Auteurs : John J. Zaunders ; Laure Moutouh-De Parseval ; Shinichi Kitada ; John C. Reed ; Steffney Rought [États-Unis] ; Davide Genini [États-Unis] ; Lorenzo Leoni [États-Unis] ; Anthony Kelleher [Australie] ; David A. Cooper [Australie] ; Don E. Smith [Australie] ; Pat Grey [Australie] ; Jérôme Estaquier [France] ; Susan Little [États-Unis] ; Douglas D. Richman [États-Unis] ; Jacques Corbeil [États-Unis]Source :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2003-06-01.
Descripteurs français
- Pascal (Inist)
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- topic : Homme.
English descriptors
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Abstract
We measured apoptosis of subsets of T lymphocytes by single-cell analysis of caspase activation, to confirm high turnover of chemokine receptor CCR5+ T cells in subjects with acute, primary human immunodeficiency virus type 1 (HIV-1) infection (PHI). High levels of spontaneous apoptosis, consisting mainly of CD8+ T lymphocytes, were closely associated with increases in the activation markers Ki-67, CD38, and the HIV coreceptor CCR5 and with decreases in Bcl-2 and the interleukin (IL)-7 receptor at the single-cell level. Increased expression of Ki-67 and CCR5 ex vivo, as well as increased apoptosis, was seen in all T cell receptor β-chain variable region (TCRBV) subfamilies studied. The addition of IL-2 or IL-15, but not IL-7, significantly inhibited caspase activation, increased Bcl-2 expression, and rapidly initiated proliferation in vitro of CD8+ T cells expressing CCR5 and multiple TCRBV subfamilies. Furthermore, IL-15 receptor α-chain messenger RNA levels were increased in peripheral blood mononuclear cells during PHI. These results suggest that CCR5+Ki-67+Bcl-2dim activated T cells generated during PHI traffic via blood to tissue sites, where the cells may survive and/or further proliferate under the local influence of IL-2 or IL-15. Understanding cytokine effects on CCR5+ T cells will be important in understanding chronic HIV-1 replication and pathogenesis
Url:
DOI: 10.1086/375030
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Zaunders</name><affiliation><wicri:noCountry code="subField">Hospital</wicri:noCountry></affiliation></author><author><name sortKey="Moutouh De Parseval, Laure" sort="Moutouh De Parseval, Laure" uniqKey="Moutouh De Parseval L" first="Laure" last="Moutouh-De Parseval">Laure Moutouh-De Parseval</name><affiliation><wicri:noCountry code="no comma">Centre for AIDS Research</wicri:noCountry></affiliation></author><author><name sortKey="Kitada, Shinichi" sort="Kitada, Shinichi" uniqKey="Kitada S" first="Shinichi" last="Kitada">Shinichi Kitada</name><affiliation><wicri:noCountry code="subField">Jolla</wicri:noCountry></affiliation></author><author><name sortKey="Reed, John C" sort="Reed, John C" uniqKey="Reed J" first="John C." last="Reed">John C. 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Cooper</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>National Centre in HIV Epidemiology and Clinical Research, University of New South Wales, Darlinghurst, New South Wales</wicri:regionArea><wicri:noRegion>New South Wales</wicri:noRegion></affiliation></author><author><name sortKey="Smith, Don E" sort="Smith, Don E" uniqKey="Smith D" first="Don E." last="Smith">Don E. Smith</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>National Centre in HIV Epidemiology and Clinical Research, University of New South Wales, Darlinghurst, New South Wales</wicri:regionArea><wicri:noRegion>New South Wales</wicri:noRegion></affiliation></author><author><name sortKey="Grey, Pat" sort="Grey, Pat" uniqKey="Grey P" first="Pat" last="Grey">Pat Grey</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>National Centre in HIV Epidemiology and Clinical Research, University of New South Wales, Darlinghurst, New South Wales</wicri:regionArea><wicri:noRegion>New South Wales</wicri:noRegion></affiliation></author><author><name sortKey="Estaquier, Jerome" sort="Estaquier, Jerome" uniqKey="Estaquier J" first="Jérôme" last="Estaquier">Jérôme Estaquier</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM CJF 97-07, Groupe Hopitalier Claude-Bernard Bichat, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Little, Susan" sort="Little, Susan" uniqKey="Little S" first="Susan" last="Little">Susan Little</name><affiliation></affiliation><affiliation wicri:level="3"><country>États-Unis</country><placeName><settlement type="city">San Diego</settlement><region type="state">Californie</region></placeName><wicri:orgArea>Department of Medicine, University of California</wicri:orgArea></affiliation><affiliation wicri:level="3"><country>États-Unis</country><placeName><settlement type="city">San Diego</settlement><region type="state">Californie</region></placeName><wicri:orgArea>San Diego Veterans Affairs Healthcare System</wicri:orgArea></affiliation></author><author><name sortKey="Richman, Douglas D" sort="Richman, Douglas D" uniqKey="Richman D" first="Douglas D." last="Richman">Douglas D. Richman</name><affiliation></affiliation><affiliation wicri:level="3"><country>États-Unis</country><placeName><settlement type="city">San Diego</settlement><region type="state">Californie</region></placeName><wicri:orgArea>Department of Medicine, University of California</wicri:orgArea></affiliation><affiliation wicri:level="3"><country>États-Unis</country><placeName><settlement type="city">San Diego</settlement><region type="state">Californie</region></placeName><wicri:orgArea>San Diego Veterans Affairs Healthcare System</wicri:orgArea></affiliation></author><author><name sortKey="Corbeil, Jacques" sort="Corbeil, Jacques" uniqKey="Corbeil J" first="Jacques" last="Corbeil">Jacques Corbeil</name><affiliation></affiliation><affiliation wicri:level="3"><country>États-Unis</country><placeName><settlement type="city">San Diego</settlement><region type="state">Californie</region></placeName><wicri:orgArea>Department of Medicine, University of California</wicri:orgArea></affiliation><affiliation wicri:level="3"><country>États-Unis</country><placeName><settlement type="city">San Diego</settlement><region type="state">Californie</region></placeName><wicri:orgArea>San Diego Veterans Affairs Healthcare System</wicri:orgArea></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Reprints or correspondence: Dr. Jacques Corbeil, Dept. of Medicine, University of California, San Diego, 9500 Gilman Dr., La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation></author></analytic><monogr></monogr><series><title level="j">The Journal of Infectious Diseases</title><title level="j" type="abbrev">The Journal of Infectious Diseases</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><publisher>The University of Chicago Press</publisher><date type="published" when="2003-06-01">2003-06-01</date><biblScope unit="volume">187</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="1735">1735</biblScope><biblScope unit="page" to="1747">1747</biblScope></imprint><idno type="ISSN">0022-1899</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1899</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>AIDS</term><term>Apoptosis</term><term>CCR5 chemokine receptor</term><term>Cell proliferation</term><term>HIV-1 virus</term><term>Human</term><term>Interleukin 15</term><term>Interleukin 2</term><term>Regulation</term><term>T-Lymphocyte</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Apoptose</term><term>Homme</term><term>Interleukine 15</term><term>Interleukine 2</term><term>Lymphocyte T</term><term>Multiplication cellulaire</term><term>Protéine Bcl2</term><term>Récepteur chimiokine CCR5</term><term>Réglementation</term><term>SIDA</term><term>Virus HIV1</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">We measured apoptosis of subsets of T lymphocytes by single-cell analysis of caspase activation, to confirm high turnover of chemokine receptor CCR5+ T cells in subjects with acute, primary human immunodeficiency virus type 1 (HIV-1) infection (PHI). High levels of spontaneous apoptosis, consisting mainly of CD8+ T lymphocytes, were closely associated with increases in the activation markers Ki-67, CD38, and the HIV coreceptor CCR5 and with decreases in Bcl-2 and the interleukin (IL)-7 receptor at the single-cell level. Increased expression of Ki-67 and CCR5 ex vivo, as well as increased apoptosis, was seen in all T cell receptor β-chain variable region (TCRBV) subfamilies studied. The addition of IL-2 or IL-15, but not IL-7, significantly inhibited caspase activation, increased Bcl-2 expression, and rapidly initiated proliferation in vitro of CD8+ T cells expressing CCR5 and multiple TCRBV subfamilies. Furthermore, IL-15 receptor α-chain messenger RNA levels were increased in peripheral blood mononuclear cells during PHI. These results suggest that CCR5+Ki-67+Bcl-2dim activated T cells generated during PHI traffic via blood to tissue sites, where the cells may survive and/or further proliferate under the local influence of IL-2 or IL-15. Understanding cytokine effects on CCR5+ T cells will be important in understanding chronic HIV-1 replication and pathogenesis</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li><li>États-Unis</li></country><region><li>Californie</li><li>Île-de-France</li></region><settlement><li>Paris</li><li>San Diego</li></settlement></list><tree><noCountry><name sortKey="Kitada, Shinichi" sort="Kitada, Shinichi" uniqKey="Kitada S" first="Shinichi" last="Kitada">Shinichi Kitada</name><name sortKey="Moutouh De Parseval, Laure" sort="Moutouh De Parseval, Laure" uniqKey="Moutouh De Parseval L" first="Laure" last="Moutouh-De Parseval">Laure Moutouh-De Parseval</name><name sortKey="Reed, John C" sort="Reed, John C" uniqKey="Reed J" first="John C." last="Reed">John C. Reed</name><name sortKey="Zaunders, John J" sort="Zaunders, John J" uniqKey="Zaunders J" first="John J." last="Zaunders">John J. Zaunders</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Rought, Steffney" sort="Rought, Steffney" uniqKey="Rought S" first="Steffney" last="Rought">Steffney Rought</name></region><name sortKey="Corbeil, Jacques" sort="Corbeil, Jacques" uniqKey="Corbeil J" first="Jacques" last="Corbeil">Jacques Corbeil</name><name sortKey="Corbeil, Jacques" sort="Corbeil, Jacques" uniqKey="Corbeil J" first="Jacques" last="Corbeil">Jacques Corbeil</name><name sortKey="Corbeil, Jacques" sort="Corbeil, Jacques" uniqKey="Corbeil J" first="Jacques" last="Corbeil">Jacques Corbeil</name><name sortKey="Corbeil, Jacques" sort="Corbeil, Jacques" uniqKey="Corbeil J" first="Jacques" last="Corbeil">Jacques Corbeil</name><name sortKey="Corbeil, Jacques" sort="Corbeil, Jacques" uniqKey="Corbeil J" first="Jacques" last="Corbeil">Jacques Corbeil</name><name sortKey="Genini, Davide" sort="Genini, Davide" uniqKey="Genini D" first="Davide" last="Genini">Davide Genini</name><name sortKey="Genini, Davide" sort="Genini, Davide" uniqKey="Genini D" first="Davide" last="Genini">Davide Genini</name><name sortKey="Leoni, Lorenzo" sort="Leoni, Lorenzo" uniqKey="Leoni L" first="Lorenzo" last="Leoni">Lorenzo Leoni</name><name sortKey="Leoni, Lorenzo" sort="Leoni, Lorenzo" uniqKey="Leoni L" first="Lorenzo" last="Leoni">Lorenzo Leoni</name><name sortKey="Little, Susan" sort="Little, Susan" uniqKey="Little S" first="Susan" last="Little">Susan Little</name><name sortKey="Little, Susan" sort="Little, Susan" uniqKey="Little S" first="Susan" last="Little">Susan Little</name><name sortKey="Richman, Douglas D" sort="Richman, Douglas D" uniqKey="Richman D" first="Douglas D." last="Richman">Douglas D. Richman</name><name sortKey="Richman, Douglas D" sort="Richman, Douglas D" uniqKey="Richman D" first="Douglas D." last="Richman">Douglas D. Richman</name><name sortKey="Rought, Steffney" sort="Rought, Steffney" uniqKey="Rought S" first="Steffney" last="Rought">Steffney Rought</name></country><country name="Australie"><noRegion><name sortKey="Kelleher, Anthony" sort="Kelleher, Anthony" uniqKey="Kelleher A" first="Anthony" last="Kelleher">Anthony Kelleher</name></noRegion><name sortKey="Cooper, David A" sort="Cooper, David A" uniqKey="Cooper D" first="David A." last="Cooper">David A. Cooper</name><name sortKey="Grey, Pat" sort="Grey, Pat" uniqKey="Grey P" first="Pat" last="Grey">Pat Grey</name><name sortKey="Smith, Don E" sort="Smith, Don E" uniqKey="Smith D" first="Don E." last="Smith">Don E. Smith</name></country><country name="France"><region name="Île-de-France"><name sortKey="Estaquier, Jerome" sort="Estaquier, Jerome" uniqKey="Estaquier J" first="Jérôme" last="Estaquier">Jérôme Estaquier</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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